Gene description for CD63
Gene name	CD63 molecule
Gene symbol	CD63
Other names/aliases	AD1 HOP-26 ME491 MLA1 OMA81H Pltgp40 TSPAN30
Species	Homo sapiens
Experiment description of studies that identified CD63 in T cells
1 Experiment ID 18 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method FACS PubMed ID 11907077    Organism Homo sapiens Experiment description TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex. Authors Blanchard N, Lankar D, Faure F, Regnault A, Dumont C, Raposo G, Hivroz C Journal name JIMMU Publication year 2002 Sample T cells Sample name Jurkat T cell blasts Isolation/purification methods Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study FACS EV-TRACK -
2 Experiment ID 91 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Immunofluorescence Western blotting FACS PubMed ID 21252909    Organism Homo sapiens Experiment description Diacylglycerol kinase a regulates the formation and polarisation of mature multivesicular bodies involved in the secretion of Fas ligand-containing exosomes in T lymphocytes Authors Alonso R, Mazzeo C, Rodriguez MC, Marsh M, Fraile-Ramos A, Calvo V, Avila-Flores A, Merida I, Izquierdo M. Journal name CDD Publication year 2011 Sample T cells Sample name J-HM1-2.2 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Lipids Methods used in the study Western blotting FACS Immunofluorescent microscopy EV-TRACK EV110031: EV-METRIC:22%
3 Experiment ID 115 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 21505438    Organism Homo sapiens Experiment description Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells. Authors Mittelbrunn M, Gutiéez-Váuez C, Villarroya-Beltri C, Gonzáz S, Sáhez-Cabo F, Gonzáz MÁ Bernad A, Sáhez-Madrid F. Journal name NATCOM Publication year 2011 Sample T cells Sample name J77 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Mass spectrometry Microarray EV-TRACK EV110049: EV-METRIC:11%
4 Experiment ID 167 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Western blotting PubMed ID 21859146    Organism Homo sapiens Experiment description Identification of a conserved glycan signature for microvesicles Authors Batista BS, Eng WS, Pilobello KT, Hendricks-MuñKD, Mahal LK Journal name J Proteome Res Publication year 2011 Sample T cells Sample name Jurkat Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV110033: EV-METRIC:11%, 13%
5 Experiment ID 168 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Western blotting PubMed ID 21859146    Organism Homo sapiens Experiment description Identification of a conserved glycan signature for microvesicles Authors Batista BS, Eng WS, Pilobello KT, Hendricks-MuñKD, Mahal LK Journal name J Proteome Res Publication year 2011 Sample T cells Sample name SupT1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV110033: EV-METRIC:11%, 13%
6 Experiment ID 169 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Western blotting PubMed ID 21859146    Organism Homo sapiens Experiment description Identification of a conserved glycan signature for microvesicles Authors Batista BS, Eng WS, Pilobello KT, Hendricks-MuñKD, Mahal LK Journal name J Proteome Res Publication year 2011 Sample T cells Sample name H9 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV110033: EV-METRIC:11%, 13%
7 Experiment ID 214 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting Immunoelectron microscopy PubMed ID 21364924    Organism Homo sapiens Experiment description Thermal- and oxidative stress causes enhanced release of NKG2D ligand-bearing immunosuppressive exosomes in leukemia/lymphoma T and B cells Authors Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L Journal name PLoS One Publication year 2011 Sample T cells Sample name Jurkat Isolation/purification methods Differential centrifugation Ultracentrifugation Filtration Sucrose density gradient FACS Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Immunoelectron microscopy FACS EV-TRACK EV110095: EV-METRIC:11%
8 Experiment ID 698 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method FACS PubMed ID 28811610    Organism Homo sapiens Experiment description Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA. Authors Néth A, Orgovan N, Só BW, Osteikoetxea X, Páczi K, Szabóylor KÉ Vukman KV, Kittel Á TuriáL, Wiener Z, TóS, Drahos L, Véy K, Horvath R, BuzáEI. Journal name Sci Rep Publication year 2017 Sample T cells Sample name Jurkat cells - Drug treated Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study FACS EV-TRACK EV170063: EV-METRIC:75%, 75%
9 Experiment ID 699 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method FACS PubMed ID 28811610    Organism Homo sapiens Experiment description Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA. Authors Néth A, Orgovan N, Só BW, Osteikoetxea X, Páczi K, Szabóylor KÉ Vukman KV, Kittel Á TuriáL, Wiener Z, TóS, Drahos L, Véy K, Horvath R, BuzáEI. Journal name Sci Rep Publication year 2017 Sample T cells Sample name Jurkat cells - Untreated Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study FACS EV-TRACK EV170063: EV-METRIC:75%, 75%
10 Experiment ID 1008 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 23463506    Organism Homo sapiens Experiment description The intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes. Authors Perez-Hernandez D, Gutiéez-Váuez C, Jorge I, Ló-MartíS, Ursa A, Sáhez-Madrid F, Váuez J, Yáz-Mó Journal name J Biol Chem. Publication year 2013 Sample T cells Sample name T lymphoblasts Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130110: EV-METRIC:22%
11 Experiment ID 1252 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 24939845    Organism Homo sapiens Experiment description Human T-lymphotropic virus type 1-infected cells secrete exosomes that contain Tax protein. Authors Jaworski E, Narayanan A, Van Duyne R, Shabbeer-Meyering S, Iordanskiy S, Saifuddin M, Das R, Afonso PV, Sampey GC, Chung M, Popratiloff A, Shrestha B, Sehgal M, Jain P, Vertes A, Mahieux R, Kashanchi F. Journal name J Biol Chem. Publication year 2014 Sample T cells Sample name Jurkat T-cells Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV140028: EV-METRIC:33%
12 Experiment ID 1253 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 24939845    Organism Homo sapiens Experiment description Human T-lymphotropic virus type 1-infected cells secrete exosomes that contain Tax protein. Authors Jaworski E, Narayanan A, Van Duyne R, Shabbeer-Meyering S, Iordanskiy S, Saifuddin M, Das R, Afonso PV, Sampey GC, Chung M, Popratiloff A, Shrestha B, Sehgal M, Jain P, Vertes A, Mahieux R, Kashanchi F. Journal name J Biol Chem. Publication year 2014 Sample T cells Sample name Jurkat T-cells with pACH Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV140028: EV-METRIC:33%